Journal: Nature biomedical engineering

Article Title: A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma.

doi: 10.1038/s41551-022-00919-w

Figure Lengend Snippet: Fig. 1 | Overview of multiplexed electrochemical sensor system. a, Overview of the microfluidic chip designed for an LOC sample-to-answer saliva detection of SARS-CoV-2 RNA and antibodies. (1) The user inputs saliva onto the antibody detection reservoir and a saliva and proteinase K mixture into the sample preparation reservoir, where it incubates. (2) The saliva is pumped over the PES membrane inside the reaction chamber for RNA capture and heated to denature potential reaction inhibitors. (3) The LAMP solution is then pumped from the reservoir into the reaction chamber and incubated. (4) The CRISPR mixture is pumped into the reaction chamber, incubated and then pumped over the EC sensor chip. (5) The saliva for antibody detection is pumped over the EC sensor chip. (6) After the addition of polystreptavidin-HRP and TMB, results from the EC sensor chip are read with a potentiostat. b, An exploded view of the multiplexed system, which includes a heater system, a sealed microfluidic chip and a multiplexed EC sensor chip. c, Photograph of the microfluidic system with a quarter dollar for scale.

Article Snippet: A microfluidic chip for a point-of-care diagnostic that integrated the multiplexed chip was designed using Autocad software and printed on a Formlabs Form 3B 3D SLA printer in grey resin (Version 4; Formlabs RS-F2-GPGR-04).

Techniques: Sample Prep, Membrane, Incubation, CRISPR

Journal: bioRxiv

Article Title: Investigating the coupled effects of stiffness and stretch on the trabecular meshwork cells using a hydrogel-integrated microfluidic system

doi: 10.64898/2026.04.17.717863

Figure Lengend Snippet: (A) Schematic illustration and a photograph of the hydrogel-integrated microfluidic system showing the multi-layer architecture. (B) Cross-sectional view demonstrating hydraulic pressure application through the microfluidic channel to induce equi-biaxial stretch on the integrated hydrogel.

Article Snippet: The inflatable microfluidic chip was designed in AutoCAD (Autodesk, USA) and fabricated on microscope slides (Fisherbrand 12-550-A3, Fisher Scientific, USA) using five PDMS-based layers ( ): (1) hydraulic pressure channel layer, (2) PDMS membrane layer, (3) hydrogel channel layer, (4) well layer, and (5) port layer.

Techniques:

Journal: bioRxiv

Article Title: Investigating the coupled effects of stiffness and stretch on the trabecular meshwork cells using a hydrogel-integrated microfluidic system

doi: 10.64898/2026.04.17.717863

Figure Lengend Snippet: The PDMS surface was treated with oxygen plasma to form a hydroxyl group (OH-PDMS). 10% TMSPMA was treated to form a methacrylate functional group on the surface (TMSPMA-PDMS). A Sigmacote-coated coverslip was placed on the TMSPMA-PDMS to form an instant microfluidic channel. A patterned Rubylith film was placed on the bottom of the microfluidic system. GelMA hydrogel was introduced from the center inlet to form four hydrogels simultaneously. UV was exposed from the bottom of the chip. Coverslips were removed to create a flat hydrogel surface.

Article Snippet: The inflatable microfluidic chip was designed in AutoCAD (Autodesk, USA) and fabricated on microscope slides (Fisherbrand 12-550-A3, Fisher Scientific, USA) using five PDMS-based layers ( ): (1) hydraulic pressure channel layer, (2) PDMS membrane layer, (3) hydrogel channel layer, (4) well layer, and (5) port layer.

Techniques: Clinical Proteomics, Functional Assay